Numerical Aperture (NA) Derivation
Introduction to Numerical Aperture
Numerical Aperture (NA) is a dimensionless number that characterizes the range of angles over which a lens or optical system can accept or emit light. It is a crucial parameter in optics and microscopy, providing information about the light-gathering ability and resolution of an optical system.
Derivation of Numerical Aperture
The Numerical Aperture is defined as the sine of the half-angle of the maximum cone of light that can enter or exit an optical system:
NA = n * sin(θ)
            Where:
            
            n is the refractive index of the medium between the lens and the external medium.
            
            θ is the half-angle of the maximum cone of light.
        
The half-angle (θ) can be derived from the geometry of the optical system using trigonometry. Consider a lens with a circular entrance or exit pupil. The maximum angle at which light can enter or exit is half of the angle subtended by the diameter of the lens:
sin(θ) = (r / f)
            Where:
            
            r is the radius of the lens.
            
            f is the focal length of the lens.
        
Substituting this expression for sin(θ) into the definition of NA gives the final formula:
NA = n * (r / f)
Significance and Applications
The Numerical Aperture is a critical parameter in microscopy, as it determines the resolving power and light-gathering ability of an optical system. Higher NA values result in better resolution and increased sensitivity to low-intensity signals.
Applications include biological and materials microscopy, where detailed imaging and analysis are crucial.

 
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